State law protects property owners from steep tax increases year to year. If a school board sets a tax rate that generates more than 4 percent additional revenue, voters can recall the tax increase. Depending on property values and market fluctuations, sometimes a 4 percent revenue increase can come from a lower tax rate.

However, we find it critical for high sensitivity that the melt profile contains not more than one or two melt domains. When assays are designed to type specific variants (SNP typing) we recommend fragment sizes of 80 100 bp. On the contrary, for mitochondrial genome screening, we recommend amplicons longer than 600 bp to identify homozygous variants22 containing more than one domain and with mutations located at different parts of melt domains..

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